MOTILITY TEST

Written by Wednesday, 05 April 2017 01:34
Objective: To determine whether an organism is motile.   Test Procedure and Interpretation   1. Prepare a semisolid agar medium in a test tube. 2. Inoculate with a straight wire,…

MANNITOL SALT AGAR TEST

Written by Wednesday, 05 April 2017 01:18
Objective: To determine the ability of an organism to grow in 7.5% NaCl and ferment mannitol. Test Procedure1. Streak an MSA plate with a light line of inoculum from the pure…

HEMOLYTIC REACTIONS

Written by Wednesday, 05 April 2017 00:57
Objective: Some pathogens are able to produce exoenzymes called hemolysins which lyse red blood cells and thus their action can be demonstrated on a blood agar plate. Test Procedure1. Using a sterile…

DNASE TEST

Written by Wednesday, 05 April 2017 00:10
Objective: DNase mediates the hydrolysis of DNA. Methyl Green indicator is stable at pHs above 7.5 but becomes colorless at lower pHs. The hydrolysis of DNA in the agar by…

CARBOHYDRATE FERMENTATION TESTS

Written by Tuesday, 04 April 2017 23:27
Objective: To determine the ability of an organism to ferment (degrade) a specific carbohydrate in a basal medium producing acid or acid with visible gas. The acid would change the…

AMYLASE TEST (STARCH HYDROLYSIS)

Written by Tuesday, 04 April 2017 22:48
Objective: To determine if the organism is capable of breaking down starch into maltose through the activity of the extra-cellular α-amylase enzyme. Test procedure1. Use a sterile swab or a…

DIAGNOSTIC TESTS FOR IDENTIFICATION OF BACTERIA

Written by Tuesday, 04 April 2017 22:16
Many different tests have been devised over the years for classification of microorganisms into families, genera, species and even subspecies. Some of these tests are quite simple to perform while…
Objective: To determine the ability of an organism to reduce nitrate to nitrite which is then reduced to free nitrogen gas. The nitrogen in nitrate serves as an electron acceptor.…

CATALASE TEST

Written by Monday, 03 April 2017 23:51
Objective: To test for the presence of the enzyme catalase. Test Procedure1. If the plate is refrigerated, it should be allowed to warm up to room temperature and then incubated…

BILE ESCULIN TEST

Written by Monday, 03 April 2017 23:28
Objective: To determine the ability of an organism to hydrolyze the glycoside esculin to esculetin and glucose. Esculetin reacts with iron to form a dark brown to black complex. The…
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