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OXYHEMOGLOBIN METHOD FOR ESTIMATION OF HEMOGLOBIN

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Published in Hemotology
Sunday, 16 July 2017 19:21
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OXYHEMOGLOBIN METHOD FOR ESTIMATION OF HEMOGLOBIN OXYHEMOGLOBIN METHOD FOR ESTIMATION OF HEMOGLOBIN
In this method, blood sample is mixed with a weak ammonia solution. Absorbance of this solution is measured in a spectrophotometer at 540 nm or in a photometer using a yellow-green filter. Absorbance of the test sample is compared with that of the standard solution.

This method is rapid and simple. However, no stable standard solution is available, color of oxyhemoglobin solution rapidly fades, and hemoglobin derivatives other than oxyhemoglobin are not measured.
Last modified on Sunday, 16 July 2017 19:34
Dayyal Dg.

Medical Laboratory Technicain at National Institute of Cardiovascular Diseases, Karachi. | Author/Writer/Blogger

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