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What to Know Before Buying Pipettes

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Friday, 07 October 2016 19:14
Why is it important to know the viscosity of the samples that will be aspirated / dispensed?
 
The viscosity of the sample will have a direct influence on which pipette is required. For aqueous samples which are low viscosity, an air displacement pipette is ideal. These pipettes are driven by a piston in an airtight sleeve which generates a vacuum. For more viscous or heavy liquids, a positive displacement pipette should be used. These pipettes are driven by a disposable piston which comes into direct contact with the sample.

How will samples required for PCR, ELISA, or other immunoassay applications affect which pipette should be used?
 
PCR, ELISA, and many other immunoassay methods utilize microwell plates. Microplates come in many configurations: 6, 24, 96, 384, and 1536 well plates arranged in a 2x3 matrix are typically used. In order to accommodate faster throughput with such methods, many pipette manufacturers offer multichannel pipettes. These allow for faster pipetting of multiple samples: instead of having to fill 96 wells individually, an 8 channel pipette can be used, reducing the number of aspirations and dispenses to 12.
 
How does the volume of samples being worked with influence which pipette is the best fit?
 
Pipettes come in a variety of different sizes to suit whichever volume needs are required. If you know you will always be pipetting the same volume of liquid, then a fixed volume pipette will be best. If the amount to be pipetted is changing from sample to sample, then a variable pipette will be ideal.
 

Oxford Step-Up To Medicine Video Series 2016

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Thursday, 14 July 2016 18:30
Abdominal and pelvic masses manifest as swellings in specific areas of the abdomen and pelvis. They are often incidentally picked up on physical examination. Most causes of abdominal masses are benign, but some might indicate a serious underlying pathology.
 
This video will provide a comprehensive diagnostic approach to a patient with an abdominal/pelvic mass. Critical questions to ask in history, such as intercurrent symptoms of change in bowel habit or urinary difficulties, and important features on physical examination, such as reducible masses and midline pulsatile masses, will be reviewed. Laboratory and imaging investigations, while not absolutely necessary, can be ordered and the significance of findings on these will also be discussed. Lastly, you will learn about the management of some serious causes of abdominal masses, such as abdominal aortic aneurysm and hernias.
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NICVD-Rehabilitation Program

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Friday, 29 April 2016 19:08
NICVD was established in 1963 and has grown from the 20-bed and now 500-bed hospital, serving more than 40,000 patient every year. NICVD has come a long way from a mere 3 floor building to a well-equipped and multi-functional health facility catering to both treatment and research needs of heart care in Pakistan.
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Quality Control Associate

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Saturday, 23 April 2016 00:39
A quality control (QC) associate carries out testing and analysis to ensure that biomedical research studies and biomedical products meet specifications and regulatory guidelines. QC associates work in quality control laboratories and in biomanufacturing environments, using complex instrumentation and laboratory equipment to conduct tests and analyses that are used in product quality regulations or scientific research studies. Because the products being developed or manufactured directly impact people’s lives, strict Food and Drug Administration regulations must be followed and documented at every step. In the manufacturing of pharmaceutical products, companies are required to follow SOPs (standard operating procedures) and every step of every process must have a traceable, written record. QC associates are responsible for this documentation. The QC associate performs analytical tests, gathers and assesses data from those tests and writes documentation and reports.

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